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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Dev Biol. 2007 Mar 24;306(1):241–254. doi: 10.1016/j.ydbio.2007.03.024

Figure 4.

Figure 4

Localization of P-Tyr proteins in the region of the meiotic spindle. Oocytes were collected from the same replicates as shown in Figure 1. Panel A is a compression of 3 serial confocal images showing the overlying cortex and the full length spindle with a small spot of P-Tyr label visible at the bottom spindle pole (Panel A). Panels B and C are two individual scans of a single MII spindle showing P-Tyr specific labeling at both spindle poles, top (B) and bottom (C). Panel D is an MII spindle from a second egg in which the layers have been compressed to allow visualization of both spindle poles on a single image. Panels A-C were co-labeled with YOL 1/34 anti-tubulin and Alexa-568 (red) to show spindle microtubules, while Panel D co-labeled with Alexa-568-Phalloidin to identify f-actin. Magnification is indicated by the bar which represents 10μm.