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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Dev Biol. 2007 Mar 24;306(1):241–254. doi: 10.1016/j.ydbio.2007.03.024

Figure 6.

Figure 6

Detection of Src-family PTKs by Western blot of mouse oocytes. Samples of unfertilized, cumulus-free oocytes were loaded on multiple lanes of a 10% SDS-PAGE gel (7.5eggs/lane) and electrophoresed, then blotted to a nylon membrane and blocked with TTBS+5% dried milk containing phosphatase inhibitors as described in “Materials and Methods”. Lanes were incubated with a control mouse monoclonal IgG (lane A), clone 28 IgG + 1mM blocking peptide (lane B), or clone 28 IgG (lane C), at a concentration of 1μg/ml overnight. The blots were then washed, incubated with goat anti-mouse IgG-peroxidase, and bound antibody was localized by chemiluminescence. The position of molecular weight standards is indicated (in KDa) at left, and the position of the Src-family PTK(s) detected with the clone 28 antibody is indicated by the arrow at right.