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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Dev Biol. 2007 Mar 24;306(1):241–254. doi: 10.1016/j.ydbio.2007.03.024

Figure 7.

Figure 7

Active Src-family PTKs associate with spindle microtubules. Oocytes and zygotes fixed at different stages of zygote development were labeled with a monoclonal antibody against activated Src-family PTKs (clone 28), or with the Nonphospho-Src(Tyr416)antibody specific for inactive Src-family PTKs (Panel C only). Bound antibody was detected with Alexa-488-goat anti-mouse IgG (green) and Hoechst 33258 (white) was used to demonstrate chromatin. Active SFKs were detected as a low level of uniform fluorescence in the cytoplasm of oocytes as well as zygotes up to the early pronuclear stage. In contrast, high levels of both activated SFKs (panel A,B) and inactive SFKs (panel C) were associated with the spindle microtubules of the unfertilized MII oocytes indicating that the spindle is associated with a population of SFKs, some of which are active. Active Src-family PTKs detected with the clone 28 antibody remained associated with the spindle from early anaphase (Panel D) and telophase (Panel E). When the male and female pronuclei formed, the active SFKs became uniformly distributed in the cytoplasm again (Panel F). Specificity was demonstrated by incubating the sample with the clone 28 antibody in the presence of the synthetic peptide (EPQYQPGENL) at 1mM (G). Magnification is indicated by the bar which represents 10μm.