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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: J Neurochem. 2008 Apr 10;106(2):591–602. doi: 10.1111/j.1471-4159.2008.05409.x

Fig. 5. MEK1/2 pathway mediates 4HPR-induced neuronal differentiation of ARPE-19 cells.

Fig. 5

Cultured ARPE-19 cells were pretreated with 1 μM U0126 for 1 h followed by incubation with 1μM 4HPR for additional 72 h. Cell lysates were prepared, and then analyzed by Western blotting using non-phospho or phospho-specific antibodies of MAPK/ERK pathway as described under Materials and Methods. Panel A, 4HPR-induced phosphorylation of c-Raf and MEK1/2 were not blocked by U0126. Panel B, the inhibition of 4HPR-induced phosphorylation of p90RSK and c-Fos by U0126. Panel C, the inhibition of 4HPR-induced phosphorylation of SAPK/JNK and c-Jun by U0126.