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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Immunity. 2009 Feb 5;30(2):218–227. doi: 10.1016/j.immuni.2008.11.015

Figure 4. Elimination of DC by diphtheria toxin in CD40L−/− and CD11cDTR mixed bone marrow chimeras reduces CTL killing.

Figure 4

Chimeric mice were generated by using CD11cDTR BM and CD40L−/− BM. Diptheria toxin (DT) was administered to one group (n=5) prior to immunization and compared to untreated mice (n=5). Mice were treated with 0.5mg GK1.5 prior to immunizing with 2×107 irradiated OVA-coated spleen cells I.V. and 0.1mg anti-CD40 (FGK45). After 7 days, 2×107 CFSEhigh labelled peptide pulsed cells and CFSElow labelled control cells in equal numbers were injected I.V. Spleens were analyzed 24 hours later. Percentage lysis was calculated by the reduction in the peptide pulsed target cells compared to unprimed mice. In (b) DC were enriched using a nycodenz gradient and stained for CD11c. As DTR was made as a fusion of DTR and GFP, DC from CD11cDTR mice express GFP. Thus the DC comprise 35% CD11cDTR and 65% CD40L−/−. Moreover, the GFP+ CD11cDTR DC are depleted upon administration of DT.