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. 2009 May 5;4:15. doi: 10.1186/1749-8104-4-15

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Copyright © 2009 Das et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Retinal ganglion cells (RGCs) are overproduced in the Ccnd1^-/-retina. (A-H) Wild-type (wt) and Ccnd1^-/-retinas were stained with an antibody against POU4F2, which marks a majority of RGCs, at E11, E12, E14.5 and P0. Arrows in (H) mark the extra layer of RGCs in the Ccnd1^-/-retina at P0. (I) Quantification of relative proportions of POU4F2⁺RGCs. (J) Quantification of relative rate of POU4F2⁺RGC production between E13.5 to E14.5. Abbreviations: DCL, differentiated cell layer; L, lens; NBL, neuroblast layer; NR, neural retina. Scale bars: 100 μm; (G) is representative for (C, G); (H) for (A, B, D, E, F, H).