Figure 6.

Patching Nlg1 did not affect non-synaptic NMDA receptor distribution. (A) The localization and density of NR1 clusters (red) was not altered by patching of transfected GFP-Nlg1 (green). NR1 was colocalized with GFP-Nlg1 clusters before and after patching (arrowheads). Scale bar, 10 μm. (B) Patching with β-NRX-Fc (red) also did not alter the already high colocalization of Nlg with NR1 (green). Scale bar, 5 μm. (C) Patching with β-NRX-Fc did not significantly change the density of non-synaptic NR1 clusters in cortical neurons or neurons treated with 2-bromopalmitate. (D) Patching of Nlg1 increased the intensity of NR1 at synaptic sites (*P < 0.05) and this increase was prevented by 2-bromopalmitate. Error bars are standard error.