Skip to main content
. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Growth Factors. 2008 Oct;26(5):254–262. doi: 10.1080/08977190802291667

Figure 5. Effect of TGF-β1 treatment on caspase-3, ERK1/2, AKT and SMAD2 activation in colorectal cancer cell lines deprived from anchorage.

Figure 5

Immunoblot assay results for caspase-3, ERK1/2, AKT and SMAD2 and their active forms in FET, Moser, GEO, and CBS maintained in suspension for 20 hours with exogenous TGF-β1 (10ng/ml) are shown. Activation of SMAD2 is present in all of the cell lines, but only FET and CBS display increased ERK1/2 phosphorylation after TGF-_1 treatment.