Figure 2. WDSV rv-cyclin inhibits NF-κB transcription after TNFα treatment.
HeLa cells were co-transfected with a NF-κB-luciferase reporter vector (pNF-κB-luc), a control expression vector, pKH3 (white bars), or rv-cyclin expression vector, pKH3-rv-cyclin (black bars), and Tk promoter-Renilla vector (pRL-Tk). Cells were treated with TNFα at 24 hrs post-transfection and harvested 5 hrs later. Results are reported as mean ± standard deviation of the ratio of luciferase activity versus Renilla activity from quadruplicate wells.