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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: Virology. 2009 Jan 26;386(1):55–60. doi: 10.1016/j.virol.2008.12.026

Fig. 4. WDSV rv-cyclin does not affect binding of NF-κB to DNA.

Fig. 4

(A) Nuclear extracts from untreated or from cells treated with TNFα and with or without induced rv-cyclin expression were incubated with a 32P-labeled oligonucleotide containing a κB binding site (lanes 2–9), and the reactions separated on a 6% nondenaturing polyacrylamide gel for autoradiography. Lane 1 contains oligonucleotide probe (P) alone. Unlabeled NF-κB probe (competitor probe) was added to the reactions in lanes 6 and 8. Unlabeled Oct1 probe (nonspecific competitor probe) was added to the reactions in lanes 7 and 9. (B) Nuclear extracts from cells treated with TNFα and with or without induced rv-cyclin were incubated with the κB binding site probe and anti-NF-κB p50 (p50) (lanes 1 & 5), anti-NF-κB p65 (p65) (lanes 2 & 6), anti-rv-cyclin (rv) (lanes 3 & 7) or normal rabbit serum (nr) (lanes 4 & 8). Reaction products were separated on a 6% gel for autoradiography.