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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: Virology. 2009 Jan 26;386(1):55–60. doi: 10.1016/j.virol.2008.12.026

Fig. 6. The rv-cyclin AD interferes with the activation of transcription by GAL4-p65AD.

Fig. 6

(A) HeLa cells were transfected with a GAL4-luciferase reporter construct (pFR-luc), a vector expressing a GAL4-DNA binding domain fusion with NF-kB p65 AD amino acids 416–551 (pGAL4-p65416–551), and a control expression vector, pKH3 (white bars), or the indicated quantities of expression construct pKH3-rv-cyclin240–270 (black bars) and pRL-Tk. Results are reported as ratios of luciferase versus Renilla activity. (B) HeLa cells transfected with GAL4 reporter vector (pFR-luc), pGAL4-p65416–551 fusion construct, and control expression vector, pKH3 (white bars), pKH3-rv-cyclin240–270 wild type (WT), or pKH3-rv-cyclin240–270 mutations (V260F or V260S) (black bars) and pRL-Tk. Results are reported as mean ± standard deviation of the ratio of luciferase activity versus Renilla activity from quadruplicate wells. The data presented in this figure are representative of two independent experiments.