Table 1. Enrichment in CD4+CD25high Treg in cultures containing various RAPA concentrationsa.
% CD4+CD25high T cells | ||
range | mean±SD | |
No RAPA | 16–22 | 19±8 |
+ RAPA 1 nM | 71–79 | 76±4 |
+ RAPA 10 nM | 76–86 | 81±6 |
+ RAPA 50 nM | 85–95 | 87±6 |
+ RAPA 100 nM | 91–99 | 96±9 |
The data are results of flow cytometry analyses using 3 week cultures of CD4+CD25high T cells obtained from 6 subjects and expanded in the presence or absence of RAPA. The gate was set on the CD4+CD25high subset of cultured cells.