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. 2009 Jun 22;4(6):e5994. doi: 10.1371/journal.pone.0005994

Table 2. Suppressor activity of Treg in R0 and R culturesa.

Suppressor cells Responder cells % suppression of proliferation p valueb
R0 CD4+CD25neg 10±2.5
CD8+CD25neg 18±9
R CD4+CD25neg 80±6 p<0.0001
CD8+CD25neg 96±5 p<0.001
a

The data are means±SD of percent suppression measured in co-cultures of proliferating autologous responder cells with Treg generated in R0 or R cultures. R cultures were set up with 1 nM RAPA. Responder cells were either CD4+CD25neg or CD8+CD25neg. The co-cultures were established with cells obtained from 10 NC. The responder cell to Treg ratios were 1∶1 in all cultures.

b

The p values are for the differences in the % suppression between Treg in the R0 and R cultures.