Table 2. Suppressor activity of Treg in R0 and R culturesa.
Suppressor cells | Responder cells | % suppression of proliferation | p valueb |
R0 | CD4+CD25neg | 10±2.5 | |
CD8+CD25neg | 18±9 | ||
R | CD4+CD25neg | 80±6 | p<0.0001 |
CD8+CD25neg | 96±5 | p<0.001 |
The data are means±SD of percent suppression measured in co-cultures of proliferating autologous responder cells with Treg generated in R0 or R cultures. R cultures were set up with 1 nM RAPA. Responder cells were either CD4+CD25neg or CD8+CD25neg. The co-cultures were established with cells obtained from 10 NC. The responder cell to Treg ratios were 1∶1 in all cultures.
The p values are for the differences in the % suppression between Treg in the R0 and R cultures.