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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Hum Reprod. 2006 Aug 12;22(1):36–44. doi: 10.1093/humrep/del328

Figure 4.

Figure 4

Phosphorylation of c-Src and epidermal growth factor receptor (EGFR). (A) E-Series prostanoid-2 (EP2) sense cells were treated with a 1:250 dilution of seminal plasma for 2 min in the absence/presence of the EP2 receptor antagonist (AH6809) or chemical inhibitors of protein kinase A (4C3MQ), c-Src (PP2), EGFR kinase (AG1478) or mitogen-activated protein kinase kinase (MEK) (PD98059). Cell lysates were immunoprecipitated with an anti-phosphotyrosine antibody and immunoblotted for phosphorylated c-Src. (B) Immunoblots were stripped and reprobed for EGFR phosphorylation. The figure shows a representative blot together with semi-quantitative analysis of c-Src or EGFR phosphorylation shown as mean ± SEM from three independent experiments; b is significantly different from a (P < 0.05).