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. 2009 Jun 19;4(6):e5959. doi: 10.1371/journal.pone.0005959

Figure 8. The BMP-4-dependent hindrance of DC maturation is specific for NPCs.

Figure 8

A–C, NPCs (grey bars), but not bone marrow-derived MSCs (blue bars), or vessel associated MSAs (green bars), inhibit the up-regulation of CD80/B7.1 (A), CD86/B7.2 (B), and MHC-II (C) on DCs maturating in vitro with LPS. Almost complete recovery of the co-stimulatory molecule expression is obtained on DC/NPC only, when soluble recombinant Noggin is added to the co-culture system. Cells were co-cultured with DCs (1∶1 cell/DC ratio) in a trans-well system. White bars are not treated (NT) DCs, while black bars are control (non co-cultured) DCs. BMP-secreting ATDC5 condrogenic cells (orange bars) are used as positive control. Data were obtained from a total of n = 2 independent experiments. Data in are mean RFI over unstained (±SEM). *p≤0.05; **p≤0.005, when either control DCs are compared to NT, co-cultures are compared to control DCs or co-cultures plus Noggin are compared to co-cultures.