Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Dec 12;40(3):14. doi: 10.1051/vetres:2008052

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© INRA, EDP Sciences, 2009

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Biochemical characterization of antigen recognized by 1F1 and 3B11 mAbs. (A) Lysates from biotin-labeled alveolar macrophages were immunoprecipitated with 1F1 mAb or an irrelevant isotype-matched control mAb (Con) and analyzed by 7.5% SDS-PAGE under reducing and non-reducing conditions. Numbers in the right indicate position where MW markers ran. (B) Detection by western-blotting of the 1F1 and 3B11 antigens on alveolar macrophage extracts subjected to 7.5% SDS-PAGE under non-reducing conditions. (C) Immunoprecipitation analysis after preclearing. Lysates from biotin-labeled alveolar macrophages were immunoprecipitated with mAb 1F1 and 3B11 after preclearing twice with the mAb indicated in the table. Arrow points the specific band, numbers in the left indicate position where MW markers ran. Results are representative of three independent experiments.