Figure 6.

Anti-Sn mAb 1F1 is efficiently internalized in alveolar macrophages upon binding. Black histograms show 1F1- Alexa 488 fluorescence associated to the cells after treatments and 37 °C incubation times indicated in the figure. Cells labeled with 1F1 mAb at 4 °C in the presence of azide to block endocytosis, either untreated (maximum binding) or treated (background binding) with the low pH buffer were used as controls. Grey histograms show binding of an isotype-matched control mAb. Results are representative of three independent experiments.