Table II.

Primers and probe sequences used in the four different allelic discrimination assays were designed using GenBank M31313.

PCR mixture	Primer	MGB-probe	AA
Codon 136	136F: 5′-CTGCAGCTGGAGCAGTGGTA-3′	136Ala: 5′FAM-TCRTGgCACTTCC-3′ (300 nM)	Ala
	136R: 5′-GATAGTAACGGTCCTCATAGTCATTGC-3′	136Val: 5′VIC-CTCATGaCACTTCC-3′(200 nM)	Val
Codon 154	154F: 5′TGGCAATGACTATGAGGACCG-3′	154Arg: 5′FAM-ACTATCgTGAAAACAT-3′(120 nM)	Arg
	154R: 5′-TGGTCTGTAGTACACTTGGTTGGG-3′	154His: 5′VIC-TACTATCaTGAAAACATG-3′(200 nM)	His
Codon 171-1	171F: 5′-GTTACCCCAACCAAGTGTACTACAGA-3′	171Arg: 5′FAM-CCAGTGGATCgGTATA-3′(150 nM)	Arg
	171R: 5′-TGTTGACACAGTCATGCACAAAG-3′	171His: 5′-ACCAGTGGATCa TTAT-3′(120 nM)	His
Codon 171-2	171F: 5′-GTTACCCCAACCAAGTGTACTACAGA-3′	171Arg: 5′FAM-CCAGTGGATCgGTATA-VIC (150 nM)	Arg
	171R: 5′-TGTTGACACAGTCATGCACAAAG-3′	171Gln: 5′VIC-ACCAGTGGATCaGTATA-3′ (200 nM)	Gln