Fig. 1.

Spontaneous fusion hybrids are hyperploid and inherit chromosomal abnormalities from both parental cell lines. (A) Experimental design for using FACS or drug selection to isolate spontaneous fusion hybrids (BLm_FACS or BLm_Drug) from the coculture of Lm and Bm cells that were differentially labeled with various markers. The fluorescence dot plots for BLm_Drug and BLm_FACS represent the results after 1 round of drug selection and 4 rounds of FACS, respectively. (B) DNA content and chromosome numbers of the parental and hybrids evaluated by flow cytometric (propidium iodide staining) and karyotyping (Giemsa staining) methods (n = haploid chromosome number of MDA-MB-231). (C) Representative SKY image showing the chromosomal composition of BLm_Drug. (D) Summary of chromosomal translocations and deletions in Bm, Lm, and BLm_Drug.