Purification of MoFe and VFe proteins from A. vinelandii. (A) Shown is 7.5% native PAGE of MoFe and VFe proteins. Lane 1, 15 μg of purified MoFe protein; lane 2, 15 μg of purified VFe protein. (B) Shown is 4–20% gradient SDS/PAGE of MoFe and VFe proteins. Lane 1, 15 μg of protein standard; lane 2, 12.5 μg of purified MoFe protein; lane 3, 12.5 μg of purified VFe protein.