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U2AF65 destabilizes the stem-loop upon binding, whereas MBNL1 binds the stem-loop structure. (A) Schematic of fluorescent RNA used to probe cTNT structure. (B) Effects of wild-type or Quad Mutant (QM) U2AFA65 on cTNT fluorescent probe. (C) Effect of MBNL1 on the fluorescent cTNT probe.
