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. 2009 May 26;106(23):9280–9285. doi: 10.1073/pnas.0901184106

Fig. 4.

Fig. 4.

Impaired DNA repair in WSTF−/− MEF cells. (A) DNA replication was intact in WSTF−/− MEF cells. DNA content from wild-type (WT) and WSTF−/− (KO) mice was measured in MEFs by flow cytometry, as described in ref. 34. (B) Cell survival after DNA damage was impaired in WSTF−/− mice. Results are expressed as the mean ± SD of 6 independent experiments (*, P < 0.05; NS, not significant). (C) Western blot analysis of the indicated proteins after knock-down by siRNA. (D) WSTF ablation reduced Snf2h recruitment to Pcna after DNA damage in MEF cells. Immunoprecipitation assays were performed 1 h after MMS treatment to induce DNA damage. Western blot analyses (WB) are shown. (E and F) Aberrant recruitment of Snf2h to Pcna after DNA damage. Immunofluorescence using the indicated antibodies was performed 1 h after MMS treatment. (Scale bar, 10 μm.) (G) Schematic illustration of WSTF as a shared component of WINAC and WICH complexes.