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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Oncogene. 2007 Jan 22;26(29):4234–4242. doi: 10.1038/sj.onc.1210209

Figure 4.

Figure 4

TAFII250 increases Mdm2 levels and downregulates Mdm2 auto-ubiquitylation. (a) H1299 cells were transfected with 5 μg of Mdm2 together with increasing amounts of TAFII250 expression vector, as indicated in the figure. 20 μM of MG132 was added to one of the plates for 4 h. Total cell extracts were analysed by Western blotting using the anti-Mdm2 antibodies SMP14 and D12. (b) The transfections were performed as above but in the presence of 2 μg of the His-Ubiquitin expression vector. Additionally, the cells were treated with 20 μM MG132 for 4 h before harvest.