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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Exp Eye Res. 2007 Jul 25;85(5):617–625. doi: 10.1016/j.exer.2007.07.014

Figure 2. IDO protein is expressed by IFN-γ treated primary and metastatic uveal melanoma cells.

Figure 2

Uveal melanoma protein lysates were prepared and 10μg of protein per sample which were fractionated by electrophoresis. Separated proteins were transferred to PVDF membranes, washed and probed using IDO or α-actin specific antibodies and visualized by HRP colorimetric assay. This assay was performed on 3 separate occasions.