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. 2009 May 6;10(5):2031–2040. doi: 10.3390/ijms10052031

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — Effect of HP-β-CD on the stability of disulfide bonds. A and B are the PAGE results. The upper graph (A) was insulin without HP-β-CD, the lower graph (B) was insulin and HP-β-CD. Lanes 1-4 in (A) and (B) indicated insulin incubated with different GSH/GSSG (mM/mM) solutions with 0, 20/1, 40/1, 60/1, GSH/GSSG solution, respectively. C is the result of HPLC. ▪—▪ indicates the remaining insulin with HP-β-CD, ♦-♦ indicates the remaining insulin without HP-β-CD.