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. 2009 May 6;10(5):2041–2053. doi: 10.3390/ijms10052041

Figure 2.

Figure 2.

Cross-linking experiments of the different Tim14/Pam18s-Tim16/Pam16s complexes. A) Cross-linking of the hTim14/Pam18s-yTim16/Pam16 complex. B) Cross-linking of the yTim14/Pam18s-hTim16/Pam16s complex. C) Cross-linking products of the hTim14/Pam18s-hTim16/Pam16s complex. Cross-linking was carried out with 1 mM DSS at room temperature in a buffer containing 20 mM Na-Hepes pH 7.4, 200 mM NaCl, 100 mM KCl and 1 mM MgCl2, at a protein concentration of 15 μM. The cross-linking reactions were stopped at different times by addition of 10 μl SDS sample buffer and further boiling for 5 minutes. The cross-linking products (15 μl) were analyzed using 16% acrylamide gels. **minor amount of higher oligomeric cross-linked forms, presumably tetramers, of Tim14/Pam18s-Tim16/Pam16s. M: molecular weight markers. The Mw is indicated to the left of the gel.