Table 2.
Constructs used in this study and their abbreviations.
| Description | Amino acids included in construct | Abbreviated name | |
|---|---|---|---|
| 1 | soluble domain of human Tim14/Pam18 | 24–116 | hTim14s/Pam18s |
| 2 | soluble domain of human Tim16/Pam16 | 24–125 | hTim16s/Pam16s |
| 3 | soluble domain of yeast Tim14/Pam18* | 84–168 | yTim14s |
| 4 | soluble domain of yeast Tim16/Pam16* | 25–130 | yTim16s/Pam16s |
| 5 | yeast Tim14s/Pam18s in complex with human Tim16s/Pam16s | 84–168 | yTim14s/Pam18s-hTim16s/Pam16s |
| 24–125 | |||
| 6 | Human Tim14s/Pam18s in complex with yeast Tim16s/Pam16s | 24–116 | hTim14s/Pam18s-yTim16s/Pam16s |
| 25–130 | |||
| 7 | human Tim14/Pam18 in complex with human Tim16s/Pam16s* | 24–116 | hTim14s/Pam18s-hTim16s/Pam16s |
| 24–125 | |||
| 8 | yeast Tim14s/Pam18s in complex with yeast Tim16s/Pam16 | 84–168 | yTim14s/Pam18s-yTim16s/Pam16s |
| 25–130 | |||
| 9 | full length yeast Tim14 | 1–168 | yTim14/Pam18 |
| 10 | yeast Tim14/Pam18 in complex with yeast Tim16/Pam16 (both full length) | 1–168 | yTim14s/Pam18s-yTim16s/Pam16s |
| 1–149 | |||
*
The purification of these constructs was reported previously.