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. 2009 May 14;10(5):2136–2145. doi: 10.3390/ijms10052136

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Cytotoxicity of RADA16-I against human leukemia cells and HUVECs by MTT proliferation assay. (A) MTT assay showed that RADA16-I had no cytotoxicity for both K562 and Jurkat cells. After treated with RADA16-I solution at the maximal concentration (2mM), the relative viabilities of K562 and Jurkat cells were 101.66 ± 4.25% and 97.44 ± 5.66%, respectively. RADA16-I solutions at lower concentrations even enhanced the proliferation of cells. (B) MTT assay displayed that RADA16-I had almost no cytotoxicity against HUVECs even at 80 μM peptide concentration (92.90 ± 3.54% viability). Data shown are Means ± SD.