TABLE 2. Comparison of kinetic constants for EAH mutants to those for HPO.
Constants were calculated by nonlinear regression fits to the Michaelis-Menten equation.
| Enzyme | Substrate | Product | Km | kcat | kcat/Km |
|---|---|---|---|---|---|
| µm | S−1 | ||||
| HPOa | PSD | Solavetivol | 14.0 ± 1.9 | 2.1 ± 0.10 | 0.15 |
| EAH | PSD | Solavetivol | 9.2 ± 1.1 | 0.4 ± 0.02 | 0.04 |
| EAH, S368V | PSD | Solavetivol | 11.4 ± 1.2 | 0.6 ± 0.02 | 0.05 |
| EAH, S368V/I484V | PSD | Solavetivol | 7.6 ± 1.2 | 0.4 ± 0.02 | 0.05 |
| EAH, S368V/S482V | PSD | Solavetivol | 6.5 ± 1.2 | 0.6 ± 0.03 | 0.09 |
a
Data are from Table I; PSD, premnaspirodiene; EA, 5-epi-aristolochene.