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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6342–6352. doi: 10.4049/jimmunol.0803464

Figure 2.

Figure 2

The small molecules catalyze peptide binding to multiple DR molecules. The rate of peptide binding in the presence of increasing concentrations of J10 or J10-1 was determined for the interaction of HA-488 with DR1 and DR4 (DR1/CLIP and DR4/CLIP as input complexes, A & B, respectively) as well as the interaction of MBP-488 with DR15 (DR15/CLIP or DR15/pMBP as input complexes, C & D, respectively). Reactions were performed with DR/peptide complexes at 150 nM and fluorescent peptides at 30 nM in citrate buffer, pH 5.2 except for the assay with DR1 (200 nM DR1/CLIP and 100 nM HA-488). Initial rates of the reaction were calculated based on mP changes per minute in the FP assay (errors bars represent standard deviation of triplicate determinations). Rates of non-catalyzed peptide binding were very low for all DR/peptide complexes.