Figure 4.

Edman degradation of internal peptide fragments of FucT-VI. The 49-kDa band blotted on PVDF membrane was sequenced after partial cleavage with formic acid in vitro to overcome the N-terminal blockade. The obtained amino acid patterns of each sequencing cycle [number given (Top)] are shown in A. The amino acid stretches present in FucT-VI are given in B. C shows localization of the correlating peptides within the full-length amino acid sequence of FucT-VI. In bold are given those amino acids that match exactly with FucT-VI; in italic, the amino acids not clearly identified.