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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: J Magn Reson. 2007 Sep 20;189(2):251–279. doi: 10.1016/j.jmr.2007.09.013

Figure 3.

Figure 3

Pulse sequence for a 2D 15N-13C-13C heteronuclear correlation experiment incorporating DNP. The EPR spectrum is continuously irradiated with 4–5 watts of microwave power yielding a steady state enhanced 1H polarization that is replenished during the recycle delay of the NMR experiment. Following 1H-15N cross-polarization, magnetization is labeled with the 15N chemical shift and then transferred to the 13C spins using band-selective 15N-13C cross polarization. Further homonuclear mixing is accomplished with a dipolar recoupling sequence such as RFDR or by proton-driven spin diffusion in the presence of an R3 recoupling field (DARR/RAD) [82–85]