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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Immunity. 2008 May 1;28(5):698–709. doi: 10.1016/j.immuni.2008.03.014

Figure 5. Blocking the selection of low affinity clonotypes.

Figure 5

0.33×105 5C.C7β and 1.2×105 2B4β splenocytes were mixed and (a) stained with pMHCII tetramers for pre-immune repertoire analysis or (b) transferred into Thy1.1 syngeneic hosts. Transferred mice were immunized with 400μg PCC in IFA or MPL-based adjuvant. (a) Representative probability contours of pMHCII tetramer staining versus Vα11 for 5C.C7β (left), 2B4β (middle) and the 5C.C7β/2B4β cell mixture as a profile of cells (right) and evaluation of cell origin after cell sorting (Vα11+pMHCII+) and RT-PCR for Jβ1.2/2.5 expression representing 5CC7β and 2B4β respectively (bar graph) (b) Representative probability contours of CD44 and CD90.2 expressions (right panel) by Vα11+ Vβ3+ (left panel) cells from draining lymph nodes of recipient mice 7 days after immunization with IFA (upper panels) or MPL-based adjuvant (lower panels); single antigen-experienced PCC-specific T helper cells (Vα11+Vβ3+CD90.2+CD44hi) were sorted and the relative abundance of 5C.C7β and 2B4β T helper cells was evaluated by single-cell RT-PCR using Jβ1.2 and Jβ2.5 specific primers, respectively. Means ±SEM n=3 for each condition.