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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Immunity. 2008 May 1;28(5):698–709. doi: 10.1016/j.immuni.2008.03.014

Figure 6. TCR-independent enhancement of clonal accumulation.

Figure 6

(a) 2×105 CFSE-labeled 5C.C7αβ splenocytes were transferred into syngeneic B10.BR hosts at the time of immunization (upper panels), 3 days (middle panels) or 5 days (lower panels) after immunization with PCC and IFA or MPL-based adjuvant. Representative probability contours of pMHCII tetramer staining versus CFSE for IFA (left) and MPL (middle) immunized recipients. Total number of Vα11+CFSElopMHCIITet+ cells in draining lymph nodes of recipients immunized with IFA or MPL-based adjuvant in bar graphs (far right). (b) 2×105 5C.C7αβ splenocytes were transferred into CD90.1+ syngeneic hosts and recipients were immunized with PCC and IFA or MPL-based adjuvant. Representative probability contours of pMHCII tetramer staining versus CD90.2 for IFA(left) and MPL (middle) immunized recipients. Total number of Vα11+CD90.2+pMHCII+ cells in draining lymph nodes of recipients immunized with IFA or MPL-based adjuvant (far right), mean ±SEM n=3 p< 0.01 (**)(two-tailed Student’s t-test).