Figure 7.

Simvastatin pretreatment reduces nuclear NF-κB translocation. Healthy human volunteers inhaled 50 μg lipopolysaccharide (LPS). Simvastatin or placebo was administered for 4 days before LPS inhalation. Monocyte-derived macrophages (MDMs) were pretreated with 5 mM simvastatin or medium control for 4 hours before stimulation with pooled bronchoaveolar lavage fluid (BALF) from subjects who had been pretreated with statin or placebo. Cells were lysed at 1 hour, and relative nuclear concentrations of the p65 subunit of NF-κB were measured. Data represent duplicate readings from MDMs from three separate volunteers, *P = 0.0001 for control versus placebo BALF, **P = 0.03 for placebo BALF versus simvastatin BALF, #P = 0.03 for placebo BALF versus simvastatin + placebo BALF.