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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Neuropharmacology. 2007 Oct 7;54(2):309–318. doi: 10.1016/j.neuropharm.2007.09.013

Fig. 4.

Fig. 4

Changes in the size of the GAT1 recycling pool. A, long-term GABA treatment decreases the size of the GAT1 recycling pool. Transporters resident on the plasma membrane were labeled with biotin at 37 °C for 30 min. Drugs were presented prior to and throughout all biotinylation steps. Immunoblots show the GAT1 immunoreactivity in 0.3 volume of total cellular lysate fraction (upper panel) and one volume of surface fraction (lower panel). Data are from eight experiments. Experimental groups that were significantly different from the control group are indicated by one asterisk; groups that were significantly different (p<0.05) from the group treated with 10 μM GABA alone are indicated by two asterisks (p < 0.05). B, TTX decreases the size of GAT1 recycling pool. Cells were maintained in medium containing TTX (1 μM) and/or K+ (20 mM) for 24 hr before measuring the size of GAT1 recycling pool. Data are from six experiments. Immunoblots show the GAT1 immunoreactivity in 0.2 volume of total cellular lysate fraction (upper panel) and one volume of surface fraction (lower panel). Experimental groups that were significantly different from the control group are indicated by one asterisk; groups that were significantly different from the group treated with 1 μM TTX alone are indicated by two asterisks (p<0.05).