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. 2009 Apr 14;1(1):e00004. doi: 10.1042/AN20090005

Figure 3. Caspase 3 processing in XIAP-KO and wild-type mice.

Figure 3

Western blot analysis for caspase 3 was performed on tissue (hippocampus, A and cortex, B) from the injured hemisphere of XIAP-KO and wild-type (WT) littermates. Pro-caspase 3 is cleaved to form two active forms of caspase 3, the p19 and the p17 form. The presence of these forms in tissue from both XIAP-KO and wild-type mice show that the lack of XIAP does not affect the processing of caspase 3 in either the hippocampus or the cortex. Tissue from the right (non-injured) hippocampus and cortex show that there is a very faint increase in the cleaved form of caspase 3 in the XIAP-KO mice in response to the injury. Western blot analysis for actin is shown as a loading control although this protein, like most cellular proteins, is actually a substrate of caspase 3 and thus cleaved when caspase 3 activity is high.