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. 2009 May 20;1(2):e00010. doi: 10.1042/AN20090009

Figure 2. IL-1 activates p38 MAPK in both neurons and astrocytes, and NF-κB only in astrocytes.

Figure 2

(A) Western blot analysis of p-p38 MAPK activation by IL-1β in neurons. Hippocampal neurons were cultured for 5 days, and treated with IL-1β for 5, 10, 20, 30 and 60 min and probed for p-p38 MAPK. The blot was stripped and reprobed for total p38 MAPK (bottom panel). (B) Western blot analysis of p-IκB in astrocytes. Cultured astrocytes were treated with IL-1β for 5, 10, 20 and 30 min and probed for p-IκB. The blot was stripped and re-probed for total IκB, which was degraded after phosphorylation (bottom panel). (C) Hippocampal astrocytes were treated with IL-1β for 5, 10, 20, 30 and 60 min and probed for p-p38 MAPK. The blot was stripped and reprobed for total p38 MAPK (bottom panel).