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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Eur J Neurosci. 2008 Dec 19;29(3):437–446. doi: 10.1111/j.1460-9568.2008.06611.x

Fig. 1.

Fig. 1

Characterization of the NBCn1 antibody. (A) Immunoblot of crude plasma membranes isolated from the primary cultures of embryonic rat hippocampal neurons. Immunoblot was performed with the guinea pig NBCn1 antibody against the C-terminal 87 amino acids of rat NBCn1-B. For preabsorption, the antibody was preabsorbed with GST/NBCn1 fusion protein containing the immunogen (50 μg/mL) for 1 h before probing the membrane. (B) Immunoblot of membrane preparation from Xenopus oocytes. Oocytes were injected with cRNA encoding rat NBCn1-E or water.