Fig. 7.

Effects of NBCn1 knockdown on cytotoxicity in Mg²⁺-free medium. (A) Glutamate cytotoxicity in Mg²⁺-free medium. Neurons with siRNA control and knockdown were incubated for 1–6 h in Mg²⁺-free medium (pH 7.2) after glutamate exposure and assessed for the cytotoxicity assay (n = 5 for each). (B) Cytotoxicity in Mg²⁺-free medium without glutamate exposure. The experimental procedure was similar to the above except that neurons were exposed to 0 mM glutamate (n = 5 for each). (C) Preincubation of neurons at pH 6.3 before glutamate exposure. Neurons were incubated at pH 6.3 or 7.2 before glutamate exposure and then incubated in Mg²⁺-free medium (pH 7.2) for 2.5 h (n = 10 for pH 6.3 and n = 5 for pH 7.2). Error bars are SEM. *P < 0.05.