FIG. 7.
The difference in redox status may result in different levels of p53 activation in WT and FA cells. In WT cells, ROS attach chromosomal DNA and generate oxidative DNA damage, mainly in the form of 8-oxo-deoxyguanosine (8-oxo-dG). The damage can induce the activation of p53 by phosphorylation (for example, phospho-p53 at Ser20–p53Ser20), leading to the repair of the oxidative DNA damage. Loss of FA function leads to increased level of ROS or reduced repair of the oxidative DNA damage. Consequently, FA cells accumulate higher level of oxidative DNA damage, leading higher level of p53 activation