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. Author manuscript; available in PMC: 2009 Jun 12.
Published in final edited form as: J Mol Biol. 2008 Mar 6;378(3):565–580. doi: 10.1016/j.jmb.2008.02.066

Fig. 6.

Fig. 6

Inhibition of in vitro assembly by NYAD-1. (a) Negatively stained EM images of immature- and mature-like particles resulting from in vitro assembly of Gag (25 μM) and CA proteins (50 μM), respectively, in the presence of no peptide (a and g, control), 0.25-fold (b and h), 0.5-fold (c and i), a molar equivalent (d and j), and fivefold molar equivalent of NYAD-1 (e and k) and CAI (f and l). A dose-response effect was observed with NYAD-1. The integrity of the mature-like particles is shown in the insets. Gag in vitro assembly was conducted by dialyzing against 50 mM Na2HPO4, pH 8.0 containing 0.1 M NaCl in the presence of 5% total E. coli RNA (RNA/protein = 1:20, w/w). The CA assembly reaction was initiated at a final concentration of 1.2 M NaCl. (b) Dosage-dependent inhibition of Gag assembly; 20 fields under EM were screened and the number of VLPs was plotted against the ratio of concentration of NYAD-1 to Gag proteins in the assembly reaction.