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. Author manuscript; available in PMC: 2009 Jun 12.
Published in final edited form as: J Mol Biol. 2008 Mar 6;378(3):565–580. doi: 10.1016/j.jmb.2008.02.066

Fig. 9.

Fig. 9

Inhibitory effects of NYAD-1 in a single-cycle viral infection assay. (a and b) Dose-dependent HIV-1 inhibitory effects of NYAD-1 and reference compound, AZT. Env-pseudotyped virus particles were incubated in the presence of serially diluted NYAD-1 or AZT for 30 min or 4 h and then U-87-T4-CXCR4 cells were infected with the treated virus. Virus infectivity was determined by luciferase assay three days post-infection. (c) Time-of-addition experiments using the above-described single-cycle viral infection assay. U87-T4-CXCR4 cells were infected in the presence of 50 μM NYAD-1, 3.75 μM AZT or 222 nM T-20. The compounds were applied at different times after infection. The concentrations of compound correspond to the final concentrations of compounds when the cells were exposed to pseudoviruses.