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. Author manuscript; available in PMC: 2009 Jun 12.
Published in final edited form as: Science. 2008 Dec 19;322(5909):1843–1845. doi: 10.1126/science.1165771

Figure 2.

Figure 2

CRISPR interference requires an intact target sequence in plasmid DNA but not mRNA. (A) Disruption of the pG0400 nes target sequence with the orf142-I2 self-splicing intron, generating the conjugative plasmid pG0(I2). (B) Conjugation efficiency was measured as in Fig. 1D, using RP62a and the Δcrispr mutant LAM104 as recipients for pG0(wt) and pG0(I2). To test for interference with spliced nes mRNA, RP62a and LAM104 transconjugants were also used as donors of pG0(I2) to S. epidermidis ATCC 12228.