Figure 2.

Western-blot (WB) experiment demonstrating the effects of acute immobilization (A), chronic isolation (C), or combined stress (CA) on the levels of Cdk5, its activators p35 and p25 in the cytoplasm and nucleus of hippocampus: (A and B) Cdk5, p35, and p25 proteins in the cytoplasm (A) and nucleus (B) of hippocampus. Hippocampal lysates were resolved by SDS-PAGE and membranes were probed with respective antibodies that recognized indicated proteins and with anti-actin antibody as a loading control. (C) Immunoreactivities of Cdk5, p35, and p25 proteins in the cytoplasm and nucleus of hippocampus are given as mean±s.e.m. (as described under Materials and Methods); n=15 animals per experimental group. Statistical analysis was performed using one-way ANOVA followed by the Tukey's post hoc test and labelled as in the Fig. 1.