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. Author manuscript; available in PMC: 2009 Jun 12.
Published in final edited form as: J Neurooncol. 2008 Jul 22;90(2):181–189. doi: 10.1007/s11060-008-9656-8

Fig. 1.

Fig. 1

RNAi mediated knockdown of Pyk2 and FAK expression. SF767 glioma cells were stably transduced with a lentiviral vector expressing a shRNAi targeting Pyk2 or FAK and the DsRed fluorescent marker. (a) FACS histogram of the mass sorted cell populations. Cell number is on the ordinate and log fluorescence intensity on the abscissa. Solid histogram is wild type SF767 cells. (b) Cell lysates of wild-type SF767, SF Pyk2i, or SF FAKi cells immunoblotted with the indicated antibodies. (c) Subconfluent cultures of the indicated cell lines were serum starved for 16 h and the percentage of cells in S-phase was determined by flow cytometry 24 h after addition of serum. * P<0.05 relative to SF767 WT