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. Author manuscript; available in PMC: 2009 Jun 12.
Published in final edited form as: J Neurooncol. 2008 Jul 22;90(2):181–189. doi: 10.1007/s11060-008-9656-8

Fig. 4.

Fig. 4

Stable expression of wild-type or I308E Pyk2 FERM domain. SF767 cells were stably transduced with a lentiviral vector expressing the wild-type Pyk2 FERM domain or the I308E FERM domain variant. (a) FACS histogram of the mass sorted cell populations. Indicated cells are in the open histogram and wild type SF767 cells in the filled histogram. (b) Cell lysates of SF767 WT, SF Pyk2 FERM, or SF PF I308E cells were immunoblotted with the indicated antibodies. (c) Subconfluent cultures of the indicated cell lines were serum starved for 16 h and the percentage of cells in S-phase was determined by flow cytometry 24 h after addition of serum