Figure 6. Phenotype of RNPs with NP mutations in the NP-NP interaction site.

Recombinant RNPs were generated and amplified in vivo using either wt of mutant NP as indicated. After purification of progeny RNPs by pull-down with Ni-NTA-agarose, their accumulation was determined by Western-blot with anti-NP antibodies. (A) Results of a representative experiment, including the analysis of total cell extracts (Input) and the purified RNPs (RNPs). (B) Average and range of two experiments.