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. 2009 Jun 26;5(6):e1000494. doi: 10.1371/journal.ppat.1000494

Figure 2. Phenotype of iNOS-producing cells following Leishmania infection.

Figure 2

C57BL/6 mice were injected into the footpad with PBS or L. major promastigotes. Mice were sacrificed four weeks post-infection and the draining popliteal lymph nodes were collected. Total pooled lymph node cells from uninfected and infected mice as well as iNOS+-gated cells from infected mice were analyzed by flow cytometry for FSC, CD4, CD8α, CD11b, CD11c, CD40, CD62L, CD115, F4/80, 7/4, Ly-6C, Ly-6G, Mac3 and MHC-II expression. Results are derived from at least n = 5 mice and the data are representative of 3 independent experiments.