Table 3.
Effect of Gpa1 deficiency, Gpa1 overexpression, and Gpa1 inactivation on microtubule orientation and dynamics
| Mean length captured Mts (μm) | Distally oriented Mts (%) | Shrinkage rate (μm s−1) | Shrinkage frequency (min−1) | Shrinkage duration (s) | |
|---|---|---|---|---|---|
| Wild type, 30°C | 1.33 ± 0.16 (n = 20) | 23 ± 8 (n = 135) | 0.05 ± 0.01 (n = 42) | 0.42 ± 0.02 (n = 10) | 34 ± 3 (n = 42) |
| Gal1EG43-Gpa1 (off)a | 2.27 ± 0.28* (n = 9) | 31 ± 4++ (n = 100) | 0.03 ± 0.004 (n = 6) | 0.06 ± 0.003+ (n = 10) | 32 ± 8 (n = 6) |
| Wild type, 23°C | 1.42 ± 0.13 (n = 11) | 29 ± 10 (n = 62) | 0.02 ± 0.004 (n = 10) | 0.42 ± 0.04 (n = 10) | 66 ± 8 (n = 10) |
| Wild type, 37°C | 1.45 ± 0.25 (n = 10) | 21 ± 9 (n = 63) | 0.04 ± 0.004 (n = 10) | 0.68 ± 0.04 (n = 10) | 35 ± 5 (n = 10) |
| gpa1ts, 23°C | 1.49 ± 0.20** (n = 10) | 35 ± 11** (n = 60) | 0.02 ± 0.002 (n = 9) | 0.34 ± 0.06 (n = 10) | 52 ± 6 (n = 9) |
| gpa1ts, 37°C | 2.50 ± 0.23*** (n = 10) | 66 ± 11*** (n = 53) | 0.05 ± 0.01 (n = 10) | 0.22 ± 0.07** (n = 10) | 33 ± 6 (n = 10) |
| Gal1EG43-Gpa1 (on)b | ND | 60 ± 11* (n = 80) | ND | ND | ND |
| Gal1EG43-Gpa1 (on)b, kar3Δ | ND | 21 ± 3 (n = 78) | ND | ND | ND |
Microtubule lengths, shrinkage rates, and shrinkage durations are reported as the mean ± SEM The percentages of distally oriented microtubules (Mts) and the shrinkage frequencies are reported as the mean ± SEP.
*p ≤ 0.005, **p < 0.0005, ***p < 10−6, +p < 10−8, ++p < 10−10. The statistical comparisons are as follows: gpa1ts cells at 37°C vs. wild-type cells at 37°C; gpa1ts cells at 23°C vs. gpa1ts cells at 37°C; all others vs. wild type at 30°C. ND, not determined.
a Glucose medium.
b Galactose medium.